Background Recently, several studies have investigated the relationship between Pre\miR\27a rs895819 polymorphism and risk of various cancers
Background Recently, several studies have investigated the relationship between Pre\miR\27a rs895819 polymorphism and risk of various cancers. upregulated in DLBCL tissues compared with normal lymphoid tissues. Further in vitro experiments showed that miR\27a might function as an oncogene through target TGFBR1. In addition, TGFBR1 overexpression rescues effects of miR\27a inhibitor on DLBCL cells phenotypes. Conclusions In conclusion, these findings indicate that rs895819 A? ?G might reduce the expression of mature miR\27a, and leading a higher level of TGFBR1, ultimately inhibiting the development of DLBCL. trend.00132 Open in a separate window aAdjusted for age, gender in the logistic regression model. 3.3. Stratified analysis of rs895819 polymorphism and DLBCL risk The association between the rs895819 polymorphism and the risk of DLBCL was further evaluated by stratification analysis. The results showed the risk of DLBCL was significantly associated with in the subgroups of age 60, normal LDH, III?+?IV Ann Arbor stage, and 0\2 IPI (assessments, Error bars, SD) B, Relationship between re895819 genotypes and expression of miR\27a. (n?=?60, 35, and 5, for AA, AG, and GG, respectively, ***assessments, Error bars, SD) 3.5. Effects of miR\27a on DLBCL cell phenotypes F9995-0144 In order to seek the function of miR\27a in DLBCL, we then transfected miR\27a mimic and inhibitor into OCI\LY18 and OCI\LY19 cell. As shown in Physique ?Physique2A,2A, expression level of miR\27a in DLBCL cells was significantly increased and decreased when treated with miR\27a\3p mimic and inhibitor, respectively. Furthermore, DLBCL cells treated with miR\27a\3p inhibitor showed decreased migration (Physique ?(Physique2B),2B), invasion (Physique ?(Physique2C),2C), and proliferation ability (Physique ?(Physique2D,E),2D,E), as well as increased apoptosis rate (Physique ?(Physique2F,G).2F,G). DLBCL cells treated with miR\27a\3p mimic showed increased migration, invasion, and proliferation ability (Physique ?(Figure22B\E). Open in a separate window Physique 2 Effects of miR\27a on DLBCL cell phenotypes. A, The expression of miR\27a after transfection with miR\27a F9995-0144 mimic or inhibitor in DLBCL cells. (n?=?3 each, **tests, Error bars, SD) 3.6. Effects of miR\27a MST1R on TGFBR1 expression To understand the functional mechanism by which miR\27a modulates the malignant biological behavior of DLBCL cells, we then focused on identifying the direct targets of miR\27a using Targetscan database (http://www.targetscan.org/vert_72/). We found that 3UTR harbors a putative miR\27a binding site. To verify whether is usually a bona fide target of miR\27a, the wild and mutant type 3UTR were then constructed in the psiCHECK\2 vector (Physique ?(Figure3A).3A). The followed dual\luciferase activity assay results showed luciferase activity in those two cells were significantly inhibited after co\transfected with miR\27a\3p mimic and wild type of TGFBR1 3UTR (Physique ?(Physique3B,C)3B,C) and increased after co\transfected with miR\27a\3p inhibitor and wild type of TGFBR1 3UTR (Physique ?(Physique3D,E).3D,E). In addition, results F9995-0144 from RT\qPCR and Western blot (Physique ?(Physique3F,G)3F,G) showed that both of TGFBR1 mRNA and protein levels were downregulated in cells treated with miR\27a\3p mimic and upregulated in cell treated with miR\27a\3p inhibitor. Open in a separate window Physique 3 Effects of miR\27a on TGFBR1 regulation. A, Predicted target sites of miR\27a on TGFBR1 and the mutant sequence are shown. B,C, Relative reporter gene activity of psiCHECK\2 made up of mutant or wild type TGFBR1 3UTR co\transfected with miRNA control or miR\27a mimics in OCI\LY18 (B) and OCI\LY19 (C) cells lines. (n?=?3 each, ***tests, Error bars, SD). D,E, Relative reporter gene activity of psiCHECK\2 made up of mutant or wild type TGFBR1 3UTR co\transfected with miRNA control or miR\27a inhibitor in OCI\LY18 (D) and OCI\LY19 (E) cells lines. (n?=?3 each, **tests, Error bars, SD). F,G, Expression of TGFBR1 mRNA (F) and Protein (G) in OCI\LY18 and OCI\LY19 lines co\transfected with miRNA control, miR\27a inhibitor or inhibitor. (n?=?3 each, *** 3UTR. In addition, overexpression rescues effects of miR\27a inhibitor on DLBCL cells phenotypes. Taken together, these findings indicate the oncogenic role of miR\27a/axis in development of DLBCL. In conclusion, we have shown that rs895819 A? ?G could reduce the expression of mature miR\27a, leading upregulating of TGFBR1, and ultimately inhibiting the development of DLBCL. Undeniably, two limitations of this study should be resolved herein. First, only one stage case\control study was performed in this study, in which the sample size might not be large enough to achieve sufficient statistical power. Second, only a CHB populace\based study.