Data Availability StatementThe datasets generated for this study are available on request to the corresponding author
Data Availability StatementThe datasets generated for this study are available on request to the corresponding author. performed to diagnose or rule out biliary atresia. Serum LN, HA, and PIIINP were PF-2341066 supplier measured prior to laparoscopic PF-2341066 supplier exploration. Liver biopsy was performed for those patients. Liver fibrosis was staged on a five-point level (F0CF4) according to the METAVIR rating system. The correlation between serum markers and liver fibrosis stage was assessed. A receiver operator characteristic analysis was performed to determine the accuracy of serum markers for predicting the liver fibrosis stage. Results: Serum PIIINP and HA were positively correlated with liver fibrosis stage (= 0.622, 0.001, and = 0.41, 0.001, respectively). There was no significant correlation between serum LN and liver fibrosis stage ( 0.05). Serum aspartate aminotransferase, total bilirubin, direct bilirubin, and PIIINP were individually correlated with the fibrosis stage on multivariate ordinal regression analysis. Receiver operating curve (ROC) analysis showed that serum PIIINP was the most effective for the analysis of fibrosis grade. The area under the ROC curves (AUROCs) for serum PIIINP for diagnosing fibrosis phases F1, F2, F3, and F4 (cirrhosis) were 0.843, 0.789, 0.82, and 0.891, respectively. The cut-off serum PIIINP value for predicting fibrosis stage F1 was 242.3 ng/mL, with 73.8% sensitivity and 90% specificity. The cut-off value for predicting cirrhosis was 698.7 ng/mL, with 75% level of sensitivity and 96% specificity. Summary: Serum PIIINP is definitely a encouraging biomarker for predicting liver fibrosis stage, especially cirrhosis. Its assessment is definitely a simple and non-invasive diagnostic method for liver fibrosis in babies with cholestasis. 0.05. Results Characteristics of the Individuals From July 2016 to July PF-2341066 supplier 2019, 143 term babies with cholestasis were included, while one infant was excluded because of low birth excess weight, one because of parenteral nutrition, two because of shock or sepsis, and two because of previous hepatobiliary surgery. Therefore, 137 babies were eligible for statistical analysis, including 74 females (54.0%) and 63 males (46.0%), having a median (IQR) age of 54.0 (42.0C67.0) days. Ninety babies (65.7%) were diagnosed with biliary atresia (four combined with cytomegalovirus illness), 36 (26.3%) PF-2341066 supplier with choledochal cyst (one combined with cytomegalovirus illness), four (2.9%) with cytomegalovirus hepatitis. One infant was diagnosed with Alagille syndrome with heterozygous mutation (c.532delC) in JAG1. One infant was diagnosed with Zellweger syndrome with two heterozygous mutations in PEX26. One infant was diagnosed with COACH syndrome with two heterozygous mutations in CC2D2A. The other four infants with cholestasis were of indeterminate cause. According to the METAVIR scoring system, there were 30 F0 stage infants, 41 F1 stage infants, 26 F2 stage infants, 28 F3 stage infants, and 12 F4 stage infants. Comparisons of Parameters Between the Patients With Different Fibrosis Stages Table 1 shows the comparison of clinical characteristics and laboratory examination results in patients with different fibrosis stages. There were no statistically significant differences in gender and weight between patients of different fibrosis stages. Age tended to increase with the severity of fibrosis, especially in infants with cirrhosis ( 0.05). The proportion of biliary atresia was higher in infants with higher fibrosis stage ( 0.05). Serum levels of ALT, AST, TB, DB, GGT, PIIINP, and HA were significantly different in infants at different fibrosis stages ( 0.05). There is no factor in blood platelet serum and count LN level between the groups ( 0.05). Desk 1 Assessment of features in individuals at different fibrosis phases. = 0.409, 0.446, 0.299, 0.471, 0.247, 0.622, and 0.41, respectively, 0.005), but there is simply no significant correlation between serum LN fibrosis and level stage ( 0.05). The MannCWhitney = ?4.64, 0.001). Multivariate ordinal regression evaluation demonstrated that serum AST, TB, DB, and PIIINP had been correlated with fibrosis stage modified for age group and biliary atresia individually, as demonstrated in Desk 2. Box-plot representation of serum TB, DB, AST, and PIIINP amounts in babies at different fibrosis phases are demonstrated in Shape 1. Desk 2 Multivariate ordinal regression evaluation for serum AST, TB, DB, and PIIINP with fibrosis stage after adjusting for biliary and age atresia. thead th valign=”best” align=”remaining” rowspan=”1″ colspan=”1″ Parameter /th th valign=”best” align=”middle” rowspan=”1″ colspan=”1″ Adjusted OR (95% CI) /th th valign=”best” Rabbit polyclonal to CD24 (Biotin) align=”middle” rowspan=”1″ colspan=”1″ em P /em -worth /th /thead ALT1.0025 (0.9996C1.0055)0.09AST1.0024 (1.0004C1.0045)0.021*TB1.0083 (1.0023C1.0143)0.006*DB1.0153 (1.0062C1.0245)0.001*GGT1.0003.