´╗┐Positive markers stained for are Compact disc105 associated with PE, CD90 linked CD73 and FITC associated with APC

´╗┐Positive markers stained for are Compact disc105 associated with PE, CD90 linked CD73 and FITC associated with APC. 100,000 occasions. Body S3. Immunophenotyping of cryopreserved cells at 2?h post-thaw: Consultant histograms of expression of Compact disc90, Compact disc105, CD14 and CD73, CD20, Compact disc34, HLA-DR and Compact disc45 all associated with PerCP. Dark peaks represent isotype handles in every histograms. The initial row of histograms is perfect for M4 (crimson), the next for M6 (blue) and the 3rd for M7 (green). All measurements had been performed in triplicates from three indie experiments predicated on at least 100,000 occasions. Body S4. Immunophenotyping of cryopreserved cells at 4?h post-thaw: Consultant histograms of expression of Compact disc90, Compact disc105, Compact disc73 and Compact disc14, Compact disc20, Compact disc34, Compact disc45 and HLA-DR all associated with PerCP. Dark peaks represent isotype handles in every histograms. The initial row of histograms is perfect AG 957 for M4 (crimson), the next for M6 (blue) and the 3rd for M7 (green). All measurements had been performed in triplicates from three indie experiments predicated on at least 100,000 occasions. Body S5. Immunophenotyping of cryopreserved cells at 24?h post-thaw: Consultant histograms of expression of Compact disc90, Compact disc105, Compact disc73 and Compact disc14, Compact disc20, Compact disc34, Compact disc45 and HLA-DR all associated with PerCP. Dark peaks represent isotype handles in every histograms. The initial row of histograms is perfect for M4 (crimson), the next for M6 (blue) and the 3rd for M7 (green). All measurements had been performed in triplicates from three indie experiments predicated on at least 100,000 occasions. Body S6. Apoptosis and viability methods from the M4 cell series: Representative overlay histograms of empty samples (dark) and M4 examples stained with Annexin V and 7AAdvertisement (crimson) at different period factors. All measurements had been performed in triplicates from AG 957 three indie experiments predicated on at least 100,000 occasions. Body S7. Apoptosis and viability methods from the M6 cell series: Representative overlay histograms of empty samples AG 957 (dark) and M6 examples stained with Annexin V and 7AAdvertisement (crimson) at different period factors. All measurements had been performed in triplicates from three indie experiments predicated on at least 100,000 occasions. Body S8. Apoptosis and viability methods from the M7 cell series: Representative overlay histograms of empty samples (dark) and M7 examples stained with Annexin V and 7AAdvertisement (green) at different period factors. All measurements had been performed in triplicates from three indie experiments predicated on at least 100,000 occasions. 13287_2020_2054_MOESM1_ESM.zip (687K) GUID:?E1262AFA-831A-4262-9B28-4B638995D553 Extra document 2: Figures S9-S15. clean and cryopreserved cells stained positive for Essential oil Crimson O staining and Alizarin Crimson staining after incubation with adipogenic and osteogenic differentiation mass media. Figure S9. Essential oil Crimson O staining of clean and cryopreserved M4 cells pursuing 19 times of incubation in adipogenic differentiation mass media: Light microscopy representative pictures: (A) M4 clean control well, (B) M4 clean differentiation well, (C) M4 cryopreserved control well, (D) M4 cryopreserved differentiation well. Range bars signify 100m. Body S10. Oil Crimson O staining of clean and cryopreserved M6 cells pursuing 19 times of incubation in adipogenic differentiation mass media: Light microscopy representative pictures: (A) M6 clean control well, (B) M6 clean AG 957 differentiation well, (C) M6 cryopreserved control well, (D) M6 cryopreserved differentiation well. Range bars signify 100m. Body S11. Oil Crimson O staining of clean and cryopreserved M7 cells pursuing 19 times of incubation in adipoegnic differentiation mass media: Light microscopy representative pictures: (A) M7 clean control well, (B) M7 clean differentiation well, (C) M7 cryopreserved control well, (D) M7 cryopreserved differentiation well. Range bars signify 100m. Body S12. Alizarin Crimson staining of clean and cryopreserved M4 RFC37 cells pursuing 16 times of incubation in osteogenic differentiation mass media: Light microscopy representative pictures: (A) M4 clean control well,.

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