Supplementary MaterialsFigure 1source data 1: Caspase glo 8 measurements for IP of MPZ-GFP vs GFP. MPZ-GFP. This zip archive contains FCS files from n?=?3 biological replicates of HCT116 transfected with the conditions outlined in Determine 1D. The excel file contains the quantification of annexin V staining exported frow FlowJo. elife-52291-fig1-data3.zip (12M) GUID:?0A4659B4-6F51-4E08-9840-43BB093E2055 Figure 1source data 4: qPCR analysis of MPZ-GFP titration. This zip archive contains the compiled excel file for qPCR data shown in Physique 1figure supplement 1A along with the Prism 6 file used to perform multiple t-tests with Holm-Sidak correction for multiple comparisons. elife-52291-fig1-data4.zip (132K) GUID:?930F54D6-4627-43D2-916D-7CE30A194E4E Physique 1source data 5: Caspase glo 8 measurements for time course of MPZ-GFP transfection. This zip archive contains the measured luminescent models for caspase glo 8 activity shown in Physique 1figure supplement 1E and the tif file of the Coomassie blue-stained gel used to normalize lysate concentrations. elife-52291-fig1-data5.zip (442K) GUID:?9E1BB225-752F-4A8A-A41F-8F285729A473 Figure 1source data 6: qPCR and cell death measurement for CHOP expression. This zip archive contains the qPCR analysis from CHOP expression in Physique 1figure supplement 2B, and brightfield images of Trypan Blue staining measured around the Countess II for n?=?3 biological replicates, summarized in Determine 1figure supplement 2D. elife-52291-fig1-data6.zip (55M) GUID:?B05E551B-01F0-452A-B993-BC54C47C8DFF Physique 1source data 7: qPCR analysis of INS and RHO-GFP expression. This zip archive contains the compiled excel file for qPCR Lapatinib biological activity data shown in Physique 1figure supplement 4A along with the Prism 6 file used to perform multiple t-tests with Holm-Sidak correction for multiple comparisons. Lapatinib biological activity elife-52291-fig1-data7.zip (67K) GUID:?87ABE3C6-0660-4B8B-81C1-3C3F416E885B Physique 1source data 8: FCS files and quantification of annexin V staining for INS and RHO. This zip archive contains FCS files from n?=?3 biological replicates of HCT116 transfected with the conditions outlined in Determine 1figure supplement 4E. The excel file contains the quantification of annexin V staining exported frow FlowJo. elife-52291-fig1-data8.zip (5.5M) GUID:?7446BED3-E311-49E2-9895-883D765863DF Physique 1source Lapatinib biological activity data 9: Caspase Lapatinib biological activity glo 8 measurements for IP of INS and RHO-GFP. This zip archive contains the measured luminescent models for caspase glo 8 activity shown in Figures 1S5B (input lysates and IP beads). Coomassie gels used to normalize lysate concentration are included as. tif data files. elife-52291-fig1-data9.zip (1.0M) GUID:?A39633D1-7D65-412C-978C-E4E8C691E462 Body 2source data 1: Caspase activity for fractions of iodixanol gradient. This excel document provides the caspase glo 8 luminescent products from the fractionation examples (n?=?3 natural replicates) proven in Body 2C. elife-52291-fig2-data1.xlsx (71K) GUID:?ACF1E7DE-1E4C-469C-8939-91C89BE9DDED Body 3source data 1: Sequences and quantification of peptides probed with Fc-DR5 ECD in the peptide array. This excel document provides the peptide sequences from the peptide array proven in Body 3A, the quantification of DR5 ECD discovered for each place, and the evaluation for enriched proteins in Body 3figure dietary supplement 1. elife-52291-fig3-data1.xlsx (79K) GUID:?F06FB6B6-8864-4B21-8023-98EBDA69A378 Figure 4source data 1: Westerns and quantification of DR5 recovered on IPs. This zip archive contains?tif data files from the Westerns from inputs and IPs from the MPZ-ecto peptides (n?=?2 biological replicates) utilized Rabbit Polyclonal to ARHGEF5 to quantify the percent of DR5 recovered proven in Body 4figure dietary supplement 3A. elife-52291-fig4-data1.zip (1.4M) GUID:?2F3324BE-D82A-4276-BB0F-FE4C53261D6F Body 4source data 2: Caspase glo 8 measurements for MPZ-ecto peptide expression. This zip archive provides the assessed luminescent products for caspase glo 8 activity proven in Body 4C (lysates) as well as the coomassie gel utilized to normalize lysate focus being a.tif document. elife-52291-fig4-data2.zip (671K) GUID:?B8E5CA40-44EB-4349-A297-10D4F414FB01 Body 4source data 3: qPCR and statistical analysis for expression of MPZ-ecto peptides. This zip archive provides the put together excel apply for qPCR data proven in Body 4E combined with the Prism six document used to execute multiple t-tests with Holm-Sidak modification for multiple evaluations. elife-52291-fig4-data3.zip (78K) GUID:?8061928B-424D-41FA-88E5-322F0EA9A838 Figure 4source data 4: FCS files and quantification of annexin V staining for MPZ-ecto peptides. This zip archive contains FCS data files from n?=?3 natural replicates of HCT116 transfected using the conditions outlined in Body 4H. The excel document provides the quantification of annexin V staining exported frow FlowJo. elife-52291-fig4-data4.zip (26M) GUID:?4AF4BF8F-81FA-4244-A55F-0D32AABD4D21 Transparent reporting form. elife-52291-transrepform.pdf (140K) GUID:?25C7B4E1-DEE5-4102-B38A-0D1161EB29CC Data Availability StatementAll data have already been reported in the manuscript and accommodating files. Source documents have been supplied in all statistics. Abstract Disruption of proteins folding in.