There is a growing concern on the subject of the serious

There is a growing concern on the subject of the serious harm of radioactive materials, which are widely used in energy production, scientific study, medicine, industry and other areas. 26807-65-8 supplier post-irradiation. The results showed the selected dose of extract did not lead to acute toxicity in mice; while organizations given anthocyanins orally were significantly better than radiation control group relating to blood analysis; pretreatment of anthocyanins significantly (< 0.05) enhanced the thymus and spleen indices and spleen cell survival compared to the irradiation control group. Pretreatment with anthocyanins before irradiation significantly reduced 26807-65-8 supplier the numbers of micronuclei (MN) in bone marrow polychromatic erythrocytes (PCEs). These findings indicate that anthocyanins have immunostimulatory potential against immunosuppression induced by the radiation. < 0.01) decrease for the model group exposed to 6 Gy radiation, compared with the unirradiated control group. A significant (< 0.01) increase in the total WBC, RBC, PLT and HGB count compared to the model group was recorded for the Leucogen and ALB groups; among of them the in crease of RBC was Rabbit polyclonal to CREB1 the most highest, and was insignificantly different from the unirradiated control group (> 0.05). The peripheral blood counts in the Leucogen group were similar to those of the ALB-M group (Table 1). These results suggest that ALB could reduce the effects of radiation on hemograms in mice. Table 1 The effects of lingonberry anthocyanins on blood cell analysis of radiation in mice (mean S.D, n = 10) (**p p Normal; p p Model). Hematopoietic tissues and the immune system are highly sensitive to radiation. Radiation damage to the hematopoietic system affects the hematopoietic stem cells and reduces their cell proliferation capacity [21]. Inhibition of hematopoiesis is one of the clinical symptoms of radiation injury. Radiation injury induced by radiation is usually associated with immune system disease and a decline in white and red blood cells [22]. The results of the current study found that WBC, RBC, PLT, and total HGB in the peripheral blood of irradiated mice were increased by ALB, thus improving hematopoietic function and survival. 3.2. Effect of ALB on Immune Function Total-body irradiation of mice with 6 Gy 60Co -rays significantly reduced thymus and spleen indices (< 0.01). The spleen indices were significantly higher in all ALB-treated irradiated groups, in a dose-dependent manner, compared with the model group (< 0.01). The thymus indexes in the ALB 200 mg/kg and 100 mg/kg groups were significantly higher than that in the irradiated control group (< 0.01) (Table 2). These 26807-65-8 supplier results suggest that ALB exerted a protective effect by increasing immune organ indices. Table 2 The effects of lingonberry anthocyanins on organ/body weight ratio, phagocytic capacity of radiation in mice (mean S.D, n = 10) (**p <0.01, *p Normal; p< 0.01, p < 0.01). Combined with ALB preteatment, there were no significant differences (> 0.05) in the decrease in the rate of carbon clearance induced by radiation. ALB 50, 100, 200 mg/kg revealed more strongly an accentuation of the phagotrophy function. The rate of carbon clearance in groups treated with ALB was elevated compared with the model group (< 0.05, < 0.01), among them, the group with ALB 50 mg/kg showed the greatest PI value. 3.4. Effect of ALB on Splenocyte Proliferation To understand the immunomodulatory activity of ALB from lingonberry, we investigated its effects around the proliferation of spleenic cells. The irradiated mice groups were obviously found to display significantly decreased proliferation of splenocytes compared with that of normal control (< 0.05, < 0.01). All of the ALB test doses (50C200 mg/kg) significantly increased the proliferation of spleenic cells in a dose dependent manner compared with the model group, but less than positive control group (Physique 2). Con-A (5 g/mL) stimulated spleenocyte proliferation was significantly enhanced by Leucogen at 1.4 mg/kg and cellular proliferation was increased up to three fold in Con-A treated cells, compared to the model group. Physique 2 The effects of lingonberry anthocyanins on spleen cell proliferation of radiation in mice (mean S.D, n = 10) (**p< 0.01, *p< 0.05 Normal; #p Model). 3.5. Effect of ALB on Bone Marrow Micronucleus Formation in Mice The incidence of micronuclei (Mn) in the bone marrow was significantly increased by irradiation (< 0.01). The bone marrow micronuclei rates in the 200 mg/kg ALB, 100 mg/kg ALB and were significantly lower than in the model group, (< 0.05). The bone marrow micronuclei rate in the.

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